LOCUS BG078468 603 bp mRNA linear EST 12-MAY-2010 DEFINITION H3028B09-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3028B09 5', mRNA sequence. ACCESSION BG078468 VERSION BG078468.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 603) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG078468.1. Other_ESTs: H3028B09-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3028 row: B column: 09 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..603 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3028B09-5" /db_xref="taxon:10090" /clone="H3028B09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 181 a 105 c 125 g 192 t ORIGIN 1 gttcattata aattgtactt gaaatatatg acattttggg gtgatgtaaa taaaagtaga 61 gctcaaccct tagtagtgca gcacgtccct gagaccagtt ctaagagacg ctgaaatatt 121 gggtttaaat gaagaatcaa gaagaggctg ttggctggaa tgttagatga aacaaatctt 181 ttaaaatcaa catggggtag ggaaagagaa atcataggta cccaggcaaa tccctgagcc 241 agtaaggccc tgtagagtaa taatgaattg ttaccttaaa atcttatcac taactttatt 301 agtattatta attattacgt tggttcctac tgctgggaag ctgttcacat cgctccttat 361 acaggagcca gcaagctgta ggctttcttc agccttgctc ttcccagctc tggagagtcc 421 ctccctgacc tggtggttat aatacagcag gcggccttgg ttaatgttta ccttttcatt 481 gtgtttttga aaactacaaa tcaagtattt agtagtagct atttacaaat gtgtttgcat 541 tcgagtgctt gtagatacaa ctttacatgt aaacatgtgt aacattattt agctacattg 601 agt //