LOCUS BG078099 594 bp mRNA linear EST 12-MAY-2010 DEFINITION H3023F03-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3023F03 5', mRNA sequence. ACCESSION BG078099 VERSION BG078099.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 594) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG078099.1. Other_ESTs: H3023F03-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3023 row: F column: 03 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..594 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3023F03-5" /db_xref="taxon:10090" /clone="H3023F03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 171 a 123 c 166 g 134 t ORIGIN 1 ccggaaagat ggcggaggaa ggggaaagaa aaaaattcct ttggttccag aaaatcttct 61 gaaaaagagg aaggcttatc aagccctcaa agccactcag gcaaagcagg cacttttggc 121 aaagagagag agaaaaggaa agcaattcag gttcagacga ctagaatctt tcgtgcatga 181 ttcctggcga cagcaacgtg acaaggtgcg tgtccagcgg ctggaggtga agcctcgtgc 241 tttggaggtg cctgataaac atcccttggc cttcgttata cgcatggaaa ggattgaagg 301 agtgagttta ttggtaaaga gcaccatcat gaaacttggc ctgaagaaat tgttcagtgg 361 tgtctttgtc aaggtcaccc ctcagagtgt aagaatgctg cgcacggtgg agccttatgt 421 gacctgggga tttccaaatc tgaagtctgt ccgggagctc atcttgaaac gtggccaagc 481 aaagattaac aataagacag tccctctgac ggacaacacg gtgatcgagg agcacctggg 541 gagatttggt gtcatttgcc tggaagacct catccatgaa attgccttcc ctgg //