LOCUS BG078047 592 bp mRNA linear EST 12-MAY-2010 DEFINITION H3023A05-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3023A05 5', mRNA sequence. ACCESSION BG078047 VERSION BG078047.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 592) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG078047.1. Other_ESTs: H3023A05-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3023 row: A column: 05 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..592 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3023A05-5" /db_xref="taxon:10090" /clone="H3023A05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 170 a 141 c 157 g 124 t ORIGIN 1 gaccagaact ggtataaggc agaactcaat gggaaagatg gcttcatccc caagaattac 61 atagaaatga aaccacatcc gtggtttttt ggcaaaatcc ccagagccaa ggcagaagaa 121 atgctcagca aacagcggca tgacggggcc ttcctgatcc gagagagcga gagcgctcct 181 ggggacttct ccctgtccgt caagtttgga aatgatgtgc agcacttcaa ggtgctccgc 241 gacggagccg ggaagtattt cctgtgggtg gtgaagttta attctttgaa tgagctggta 301 gattaccaca gatcaacatc cgtgtccagg aaccagcaga tattcttacg ggacatagaa 361 cagatgccac agcagccaac ctacgtccag gcgctctttg actttgaccc ccaggaggat 421 ggcgagctgg gctttcgcag aggagacttc attcatgtca tggataactc agatcccaat 481 tggtggaaag gggcctgcca cgggcagacc ggcatgtttc cccgcaatta tgtcacccca 541 gtgaaccgga acgtctaaga agcaaaagag attatttaaa gaaagtgaaa ag //