LOCUS BG077864 585 bp mRNA linear EST 12-MAY-2010 DEFINITION H3021H08-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3021H08 5', mRNA sequence. ACCESSION BG077864 VERSION BG077864.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 585) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG077864.1. Other_ESTs: H3021H08-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3021 row: H column: 08 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..585 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3021H08-5" /db_xref="taxon:10090" /clone="H3021H08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 133 a 136 c 130 g 186 t ORIGIN 1 gagggctttg accattcgga agtaacaaga ctagctccag aatgccaagt attcgtgtaa 61 attatggtta catgttaaca ttgctgtttt tattaagcac tcatgagaat atggtgttct 121 gtatctcgaa ttccggcctt tttccagacc tctgctcacg cctgaggtat atctagcagc 181 tgtcttagtt ctagggttgc ggtagtgcat ctttgaatcc acaccaccca gggaagcagt 241 ctacatcctc tgtggtgttg cagtggtgga ttttgtgatc atggcctgtt ttactcctcc 301 tcccgtaccc cgtcctccaa atcctcaccc taccaatgca gttgttagtc tttcttctta 361 aaacctacta tggctcattt tattaaacaa gggttgtcaa cctcacatcc agacctgaca 421 cagagatctt ccagtctctg gagtcagttc ttagttcctt gtgtgggaaa ggatcgcagg 481 ggtgcatggg cgagttgcgg ggaaagctca ctccccagga gagtgaatga atagtaggtg 541 ttctttgttg actgaactct tgaaagcttt tcattttcac cattt //