LOCUS BG077774 583 bp mRNA linear EST 12-MAY-2010 DEFINITION H3019F05-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3019F05 5', mRNA sequence. ACCESSION BG077774 VERSION BG077774.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 583) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG077774.1. Other_ESTs: H3019F05-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3019 row: F column: 05 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..583 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3019F05-5" /db_xref="taxon:10090" /clone="H3019F05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 157 a 167 c 176 g 83 t ORIGIN 1 tttgctgaaa tccaccaagc ctttgaaacc atgttccagg aatccagtat ctcagatgag 61 gtggagaagg agctggggaa acgaggcacg agaggaggtg ctgggagtat gctgcaggcc 121 ccagagctgc ccaccaagac cagaacctgc aggagagcag ctgagcagaa agatgcgcct 181 gacacccctg agctgctcca cacgaagggc ctgggagaaa gcgatgcact ggacagtgag 241 ccagctgtat cgccactgct tcctcggaaa gagcgcgggc ccccagacgg cagcctaaat 301 gaagatgagc gccttctccc cagaggcaga aagaccaacc tgttcagcgc tttgatcaag 361 aagaagaaga aaatggcgcc gacgccccct aagcgcagca gttccttccg agagatggat 421 ggccagccag accgcagagg ggctagtgag gatgacagca gggaactctg caatggacca 481 ccagctctca cctcagacgc agcagagcct accaagtccc caaaggccag caatggggct 541 ggcgtcccta atggagcctt ccgggagccg ggcaactcag gct //