LOCUS       BG077672                 661 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3018D04-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3018D04 5', mRNA sequence.
ACCESSION   BG077672
VERSION     BG077672.2
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced BG077672.1.
            Other_ESTs: H3018D04-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3018  row: D  column: 04
            Seq primer: -21M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3018D04-5"
                     /db_xref="taxon:10090"
                     /clone="H3018D04"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          216 a          131 c          135 g          179 t
ORIGIN      
        1 gcgggttaaa gacactgatg atgttccaat gattctggtt ggcaataaat gcgacttgga
       61 agatgaaaga gttgtaggaa aggaacaagg tcagaaccta gcaagacagt ggaacaactg
      121 tgcattctta gaatcctctg caaagtccaa aataaatgtt aatgagatct tttatgacct
      181 agtgcggcaa attaacagaa aaactccagt gcctgggaag gcccgcaaaa agtcatcgtg
      241 tcagctgctt taatgtactg aatgcattgt agctctgagc caggtctgaa gaactgttgc
      301 ccaattcagc agtgccagca ttccaacttt gttaaaccta ccaacatctt aaacggactt
      361 tcctgtggtg gtaccctgag aggcggatga aagctactct gtcagtttgc acattctaat
      421 cactttccag tatcacgaga gatttttact tatataatag ttctagagta tgcagctggt
      481 aaaaccagag gctacatcca gtattactgc taagagacat tcttcatcca ccaatgttgt
      541 acatgtatga aaatggtgta ctgtatactt taacacaccc catactttgt attggagagt
      601 acaataatgt aaatcctaaa agcaccacta ttttagcata ataaaagaaa gtccaaagag
      661 c
//