LOCUS BG077672 661 bp mRNA linear EST 12-MAY-2010 DEFINITION H3018D04-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3018D04 5', mRNA sequence. ACCESSION BG077672 VERSION BG077672.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 661) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG077672.1. Other_ESTs: H3018D04-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3018 row: D column: 04 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..661 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3018D04-5" /db_xref="taxon:10090" /clone="H3018D04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 216 a 131 c 135 g 179 t ORIGIN 1 gcgggttaaa gacactgatg atgttccaat gattctggtt ggcaataaat gcgacttgga 61 agatgaaaga gttgtaggaa aggaacaagg tcagaaccta gcaagacagt ggaacaactg 121 tgcattctta gaatcctctg caaagtccaa aataaatgtt aatgagatct tttatgacct 181 agtgcggcaa attaacagaa aaactccagt gcctgggaag gcccgcaaaa agtcatcgtg 241 tcagctgctt taatgtactg aatgcattgt agctctgagc caggtctgaa gaactgttgc 301 ccaattcagc agtgccagca ttccaacttt gttaaaccta ccaacatctt aaacggactt 361 tcctgtggtg gtaccctgag aggcggatga aagctactct gtcagtttgc acattctaat 421 cactttccag tatcacgaga gatttttact tatataatag ttctagagta tgcagctggt 481 aaaaccagag gctacatcca gtattactgc taagagacat tcttcatcca ccaatgttgt 541 acatgtatga aaatggtgta ctgtatactt taacacaccc catactttgt attggagagt 601 acaataatgt aaatcctaaa agcaccacta ttttagcata ataaaagaaa gtccaaagag 661 c //