LOCUS BG077577 600 bp mRNA linear EST 12-MAY-2010 DEFINITION H3017A10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3017A10 5', mRNA sequence. ACCESSION BG077577 VERSION BG077577.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 600) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG077577.1. Other_ESTs: H3017A10-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3017 row: A column: 10 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..600 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3017A10-5" /db_xref="taxon:10090" /clone="H3017A10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 192 a 105 c 152 g 151 t ORIGIN 1 gtcttctaat atgggaaaac gtacctcaga agcaaccaaa gtgcatgctc cacctattaa 61 tatggaaagt gtgcacatga agattgaaga gaagatcaca ctaacctgtg ggagagatgg 121 aggattacag aatattttgt tgcatggcat gatcatgctt aggatctcag atgacaaatt 181 tggccggatt cgtcttcatg tagaaaatga agataagaaa ggggtgcagc tacagacgca 241 tccaaatgtg gataaaaaac tttttactgc agagtctctc attggcttga aaaacccaga 301 gaagtcattt ccagtcaaca gtgatgtagg ggtactaaag tggagactac aaacaacaga 361 ggaatctttt attccgttga caattaattg ctggccttcg gaaagtggaa atggctgtga 421 tgtcaacata gggtacgaac tacaggaaga taatttagag ctcaatgatg tggtcatcac 481 tatcccactc ccatcgggtg ttggcgcgcc tgtgattggt gagatcgatg gggaatatcg 541 acatgacagt cggagaaata cgttggagtg gtgcctgcca gtgattgatg ccaaaaataa //