LOCUS BG077484 606 bp mRNA linear EST 12-MAY-2010 DEFINITION H3015H10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3015H10 5', mRNA sequence. ACCESSION BG077484 VERSION BG077484.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 606) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG077484.1. Other_ESTs: H3015H10-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3015 row: H column: 10 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..606 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3015H10-5" /db_xref="taxon:10090" /clone="H3015H10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 183 a 140 c 155 g 128 t ORIGIN 1 gatgtatcac gcccccacag actgcgccac aatccggaaa tggctcacca agtgtgcaga 61 cgactccgaa acggctaact acattagtgc tcacactaaa gactgtccca agtgcaacat 121 ctgcatcgag aagaatggtt tttgtaatca catgcaatgc tccaagtgta agcacgactt 181 ctgctggatg tgtctaggag actggaagac gcatggtagt gagtactatg aatgtagtcg 241 gtacaaggag aatcctgaca ttgtcaacca gagccagcaa gcacgggcca gggaggccct 301 caagaagtac ttgttctact ttgagaggtg ggaaaatcac aacaaaagct tgcagctgga 361 ggcacaaaca tatgagcgga ttcacgagaa gattcaggag agggttatga acaacctggg 421 aacttggatt gactggcagt acctacagaa tgctgccaag ctcttagcca agtgtcgata 481 taccctgcag tacacctatc catatgcata ctacatggaa tccggtccca ggaagaagct 541 gtttgaatac cagcaggctc agctggaggc tgagatcgaa aacctttcat ggaaagttga 601 gcgtgc //