LOCUS BG077453 589 bp mRNA linear EST 12-MAY-2010 DEFINITION H3015E09-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3015E09 5', mRNA sequence. ACCESSION BG077453 VERSION BG077453.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 589) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG077453.1. Other_ESTs: H3015E09-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3015 row: E column: 09 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..589 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3015E09-5" /db_xref="taxon:10090" /clone="H3015E09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 153 a 152 c 163 g 121 t ORIGIN 1 atcccgccta ccagtgttgg ctcttcccga cccctccccc ggcgccccca gtgtccgcca 61 tggccaaagc ctacgaccac ctcttcaagt tgctgctcat cggggactcg ggggtgggca 121 agacttgtct gatcattcgc tttgcagagg acaacttcaa cagcacttac atctctacca 181 tcggaattga tttcaagatc cgaaccgtgg acatagaggg gaagaggatc aaactgcaag 241 tgtgggacac ggctggccaa gaacgattca agacaataac taccgcctat taccgtggag 301 ccatgggcat tatcctcgta tatgacatca cagatgagaa atccttcgag aatattcaga 361 actggatgaa aagcatcaaa gagaatgcct ctgcgggagt ggagcgcctc ctgctgggaa 421 acaagtgtga catggaggcc aagcggcagg tgcagagaga gcaggcggag aagttggctc 481 gagagcacag aatccgattt tttgtgacga gtgccaaatc cagtgtgaat gtggatgagg 541 ctttcagttc cctggcccgt gacatcttgc tcaagacagg aggccggag //