LOCUS       BG077398                 618 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3014H11-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3014H11 5', mRNA sequence.
ACCESSION   BG077398
VERSION     BG077398.2
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced BG077398.1.
            Other_ESTs: H3014H11-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3014  row: H  column: 11
            Seq primer: -21M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3014H11-5"
                     /db_xref="taxon:10090"
                     /clone="H3014H11"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          123 a          195 c          156 g          144 t
ORIGIN      
        1 tgtctccctg ccattggcct gtgtagagat gcccatgaac acatccggac accccaacat
       61 gcacaccttt agaaaactga ccagttgaac atgacaccat tcacagcaca aacagaactt
      121 gccgcggcac ccttgctccg ccttgcacgt aggtcatgtg acaggatctg tcggctgtca
      181 tctccgagcc gccaattgga ccaggctttg gtgtgcaagg tgcagatgct tccacacagg
      241 ctcacagcca cctggcccac ccttcctccc acaggagttc acattctttc tgactcaggt
      301 gactttccag ccccgccccc caatttctct gccctctcct gccaatcagc cgatctgggc
      361 acccatgggt gccagtggta tgtgggcagt cagggaggga gtgtcccacc actttctcag
      421 ggcagtcctt gtcttctttg tccccctccc cacccaacct gaatggcatg gctaaggatg
      481 gatgacccct cccaccttgt gggggttgaa catgaatcag gttgcacaca agtgtgtggt
      541 cctgatttgg caaggggggg ggtgcggttt gctggtttgc acaggtgccc tataggctga
      601 cagcttccta cctgagga
//