LOCUS BG077336 694 bp mRNA linear EST 12-MAY-2010
DEFINITION H3014B06-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3014B06 5', mRNA sequence.
ACCESSION BG077336
VERSION BG077336.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 694)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG077336.1.
Other_ESTs: H3014B06-3
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3014 row: B column: 06
Seq primer: -21M13 Reverse
POLYA=No.
FEATURES Location/Qualifiers
source 1..694
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3014B06-5"
/db_xref="taxon:10090"
/clone="H3014B06"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 159 a 180 c 182 g 173 t
ORIGIN
1 gaaggccttg gaccaaggac acacagctgg actggcgtgg gtgctgggag acgctgaaga
61 actgcccttc gatgacgaca gctttgatgt ttacaccatt gcatttggga tccggaatgt
121 cacacacatt gaccaggctc tccaggaagc ccatcgggtc ctaaagccag gaggccggtt
181 tctctgtctg gaatttggcc aagtgaatga ccctctcata tccaggcttt atgatctgta
241 cagcttccag gtcatccctg tcattggtga ggtcattgca ggagactgga agtcctacca
301 ataccttgtg gaaagtattc ggaagttccc aaatcaggag gacttcaagg acatgattga
361 agacgcaggc tttcagaggg tgacgtacga gaacctgact acagggattg tggccattca
421 ctctggcttc aaactctagc tccttttcta ctgtggcaca caggccagtc atttctactg
481 aaccccgggg ccagatcatc tccctgatga gacagtagct gaccgttcgt ctcctcttac
541 ggccgttaag actttggatt agctctcatt agtagctgcc caggctgctg aggccttctc
601 ccagaaatgt ctggcctggt ttctgctgga ctccagctag aggcttagcc gtgcagtgtg
661 ggagtcaggt aaaaccagca cgattgcaac tctc
//