LOCUS       BG077290                 607 bp    mRNA    linear   EST 12-MAY-2010
DEFINITION  H3013F02-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3013F02 5', mRNA sequence.
ACCESSION   BG077290
VERSION     BG077290.2
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
            Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
            Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
  TITLE     Genome-wide expression profiling of mid-gestation placenta and
            embryo using a 15,000 mouse developmental cDNA microarray
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
   PUBMED   10922068
COMMENT     On Jan 26, 2001 this sequence version replaced BG077290.1.
            Other_ESTs: H3013F02-3
            Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3013  row: F  column: 02
            Seq primer: -21M13 Reverse
            POLYA=No.
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3013F02-5"
                     /db_xref="taxon:10090"
                     /clone="H3013F02"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          159 a          160 c          150 g          138 t
ORIGIN      
        1 cctggagatc cagtcgcacc tgcggcaccc caacatcctc aggctgtatg gctatttcca
       61 tgacgccacc cgagtttatc tgattctaga atatgcgccc cttggaacag tctatagaga
      121 gctccaaaaa ctctccaagt ttgacgagca gagaacagct acttacatca ctgagttggc
      181 aaacgctctg tcttactgtc attcaaagag agtgatccac agagacatta agccagagaa
      241 cttactgctt ggctcaaacg gagagttgaa gattgcagac ttcgggtggt cggtgcatgc
      301 tccatcttcc aggagaacca caatgtgtgg caccctggac tacctgcccc cagagatgat
      361 tgaaggccgg atgcatgacg agaaggtgga cctctggagc ctcggcgttc tctgctatga
      421 gttcctagtg gggatgcctc ctttcgaggc acacacgtac caggagactt acagaaggat
      481 atctcgggtt gaattcactt tccctgactt tgtgacagag ggagccaggg acctcatttc
      541 aagactgtta aaacacaacg caagccaaag gctaacacta gcggaagtcc ttgagcaccc
      601 ttggatc
//