LOCUS BG077287 588 bp mRNA linear EST 12-MAY-2010 DEFINITION H3013E11-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3013E11 5', mRNA sequence. ACCESSION BG077287 VERSION BG077287.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 588) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG077287.1. Other_ESTs: H3013E11-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3013 row: E column: 11 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..588 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3013E11-5" /db_xref="taxon:10090" /clone="H3013E11" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 114 a 218 c 128 g 128 t ORIGIN 1 caccaagtgc ggcaactttg cacgcttcat taaccacagc tgcaatccca actgctacgc 61 caaggtgatc acggtggagt cccagaagaa gatcgtcatc tactctaagc agcacatcaa 121 cgtcaacgag gagatcacgt acgactacaa gttcccgatc gaagatgtca agatcccctg 181 cctctgtggc tccgagaact gccgggggac cctcaattag tccccgcgtg ccaaaggccg 241 aggatgtcag ccgtagccca ggagaaccct cgctcgccct gggccaggct ccacacacac 301 ccccacccac ttcaggtgct gtccttatcc ccagcagccg cactagggcc tggcgccccc 361 aacactatcc caggacccac ccgcagcccc agccccgcag cgggaaaggg cttctctgtc 421 tctccacctg cgtctttccg tttttataga tgctcttcgc acgcaggctt tgtatcctta 481 tgtatctggc tttccattct ccctccgtcc gtctgtccgt ccgtccgtct gtcttccctc 541 cctggctctg gcctcagtca tttcccccac ccctgagaat gctgctct //