LOCUS BG077159 634 bp mRNA linear EST 12-MAY-2010 DEFINITION H3011E10-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3011E10 5', mRNA sequence. ACCESSION BG077159 VERSION BG077159.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 634) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG077159.1. Other_ESTs: H3011E10-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3011 row: E column: 10 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..634 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3011E10-5" /db_xref="taxon:10090" /clone="H3011E10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 157 a 170 c 167 g 140 t ORIGIN 1 gaccagagtc cgcgaatcgt ccgctccgga cctccagacc tccccaacct cagctttctg 61 acctgtgaaa acacttaaga atcatgtctc tgcatcagtt tctgctggag ccaatcacct 121 gccatgcctg gaacagggat cgtacccaga tcgccctgag ccccaataac catgaggtgc 181 acatctataa gaagaatggg agtcagtgga cgaaagcgca cgagctgaag gaacataatg 241 ggcatatcac aggtattgac tgggctccta agagcgaccg tattgtcact tgtggggcag 301 accgcaacgc ctatgtctgg agtcagaaag atggcatctg gaagcccacc ctggtgatcc 361 tgaggattaa ccgtgcagcc acttttgtga agtggtcccc acttgagaac aagtttgctg 421 tggggagcgg agcccggctc atctctgtct gttactttga gtctgagaat gactggtggg 481 tgagcaagca cattaagaag ccgatccgct ccacagtcct cagcttggac tggcatccca 541 acaatgtttt gctggctgca ggctcctgtg acttcaaatg cagagtgttc tctgcctata 601 tcaaagaggt ggatgagaag ccagccagca cgcc //