LOCUS BG076844 617 bp mRNA linear EST 12-MAY-2010 DEFINITION H3006H01-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3006H01 5', mRNA sequence. ACCESSION BG076844 VERSION BG076844.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 617) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG076844.1. Other_ESTs: H3006H01-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3006 row: H column: 01 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..617 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3006H01-5" /db_xref="taxon:10090" /clone="H3006H01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 147 a 175 c 200 g 95 t ORIGIN 1 ggaacgccga tgcccaggta ccacatgact ccccagaacc agccgcacac ccaaatctgt 61 gtggggttga ccacgaggcg ctgagtaagc aaataatgga gtacaaacgc aagaaggagc 121 gagggcttca gtgtcagagt ctggcgccac catcagggcg ggagcaggca cctgaaccga 181 cccgtctgct ccggaagaga gagaaggtgc gatcccggtg gcagcaccgg gacgcttccc 241 ccagaagctt ccttttgggc gaggaggacg aggacgagga cagcagcaca gagtgggaat 301 gccacgccca cgaggcagag gatgatgatg atcatcagga agaggagctc ggagctgaca 361 gaggcgcaca gcggggcctc tgtggcaaga gagagcaggg gtagggctgg ggcctccgag 421 ctgccgcccc tgcctgtggg tcagactgca ggacagagcc tctggtgtac cttctgtcgt 481 agtcactcac ggaggcggcc tgacctcagc ggcaggagaa ctgcacccat cggactgcac 541 ctgcagacct ccgcagccag tcctcagcga ttggtaccta cctggtcccg gataaataaa 601 cgattttgta tttactc //