LOCUS BG076671 599 bp mRNA linear EST 12-MAY-2010 DEFINITION H3004D06-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3004D06 5', mRNA sequence. ACCESSION BG076671 VERSION BG076671.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 599) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG076671.1. Other_ESTs: H3004D06-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3004 row: D column: 06 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..599 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3004D06-5" /db_xref="taxon:10090" /clone="H3004D06" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 186 a 98 c 165 g 150 t ORIGIN 1 gggggctgtt ccgtgagaag ttctcaattt ttttcagcaa tgagtcaagt ggcagcggaa 61 agtacggccg gcctggacca gcaatttgtt ggtcttgacc tgaaatcatc agataatcag 121 aatggaggag gaaatacaga gagcaaaggg cgctatatac ctcctcactt aaggaacaga 181 gaaacatcta aaggggtctg tgataaggac agttcaggat ggagctgtag taaagataaa 241 gatgcctaca gcagttttgg atctcgtgat tccagaggga agcccaatta tttcagtgat 301 cgtggaagtg gatccagggg aaggttcgat gatcatggtc gaaatgacta tgatggtatt 361 ggtggtcgtg accgaactgg atttggcaaa tttgaacgga gtggtcatag tcgttggagt 421 gacagatcag atgaagatga ctggtcaaaa ccacttccac caagtgaacg cttagagcaa 481 gaactttttt ctggaggaaa cacagggatt aactttgaga aatatgatga tataccagta 541 gaagcaactg gaaataattg tcctcctcac attgaaaatt tcagtgatat tgagatggg //