LOCUS BG076620 608 bp mRNA linear EST 12-MAY-2010 DEFINITION H3003F11-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3003F11 5', mRNA sequence. ACCESSION BG076620 VERSION BG076620.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 608) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG076620.1. Other_ESTs: H3003F11-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3003 row: F column: 11 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..608 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3003F11-5" /db_xref="taxon:10090" /clone="H3003F11" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 177 a 149 c 170 g 112 t ORIGIN 1 ggagcgcccg gaactgcagc agtttgtagg caagagggag aagattgact ttgctgacac 61 agtcaccaag tacgacagga ggttcaaggg tgtcaagcga gacctgctgc taacccccaa 121 gtgcttgtac ttaattggaa gggagaaggt gaagcaaggc ccagacaaag gtgttgtgaa 181 ggaagtcctg aagcggagga tcgaggtgga aaggatcctg tccgtgtccc tcagtaccat 241 gcaggatgac atcttcattc tccacgagca agaatatgac agtttgctgg agtccgtctt 301 taaaacggag ttcctgagcc tcttagcaaa gcgatacgag gaaaaaaccc agaagcaact 361 acctctgaaa ttcagcaata cccttgaact gaagttgaaa aaggagaact ggggtccctg 421 gagtgcaggg ggctcccggc aagtgcagtt ccaccaaggc ttcggtgacc tggctatcct 481 caagcccagc aacaaagtgc tgcaggtcag catcggaccc gggctgccca agaactcccg 541 tcctaccaga agaaacaccg tcaccagcag agggtatcca ggtggaacta agaacaatta 601 cccgatga //