LOCUS BG076488 590 bp mRNA linear EST 12-MAY-2010 DEFINITION H3003B07-5 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3003B07 5', mRNA sequence. ACCESSION BG076488 VERSION BG076488.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 590) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG076488.1. Other_ESTs: H3003B07-3 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3003 row: B column: 07 Seq primer: -21M13 Reverse POLYA=No. FEATURES Location/Qualifiers source 1..590 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3003B07-5" /db_xref="taxon:10090" /clone="H3003B07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 139 a 187 c 178 g 86 t ORIGIN 1 gagaccaggc caggcagagg ccgaaagccg ggcagccgcc gagagcctgg ccggctagca 61 ctcaagtggg ggacacgtgg tggcttcaat ggacaaatgg aacggtcccc aagaaggact 121 caccattggc agcataatgg ggagctagcg gaagggggtg ctgagcccaa ggatccaccc 181 cttcctggga cccattctga ggaccttaag gtgcccccgg ggatagtcag aaagtctcgt 241 cgtggccgga ggagaaaata caaccctgcc cggaacagca gcagctcccg ccaggacgtg 301 accttggaac ccagccctac aacgcgagca gctgtccctt tgcctccccg ggcccgccct 361 ggacgtcctg ccaaaaataa gaggaggaaa ctggccccat agcagccata cctggagctg 421 gaatctgacc ctgactgggg agagctgagt gctgagcctt gggagcccct gccagccacg 481 tgcacctgtg gacagtgggt ggggcactac tccccactca gagcacaaat gcaacccctc 541 ccctacaaat cccatcctga gccattgcag gggtagggaa gctcacccct //