LOCUS BG076351 733 bp mRNA linear EST 12-MAY-2010 DEFINITION H3158F11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3158F11 3', mRNA sequence. ACCESSION BG076351 VERSION BG076351.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 733) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG076351.1. Other_ESTs: H3158F11-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3158 row: F column: 11 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..733 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3158F11-3" /db_xref="taxon:10090" /clone="H3158F11" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 121 a 226 c 180 g 206 t ORIGIN 1 gtaaggaccc gcagtttatt tgtgggcagc caagggtgga ggggacagtg accacacagt 61 tacttggttt tgttttcctg actatggtgg agggaagctg agacagggat ttactctgca 121 acccaggctg gactcagtct ccagatcttc tggcctctct ggctaggatc aggcacagcc 181 atagcatatg tacaagtgaa atgttgagtg tttttctgag atgcaggtca tggctctgag 241 gcaggatcat gcttaaaggc tgggactaaa ccaagagttt cttccgtcct cctcctgctc 301 ctcccctata ggacggacgc tcagaacctc ggcattcaga ggctgcagga gcatgcccag 361 gtatcctgcc ccattccacc atgtgccagc ctgggctgtg cacctcactc tgcactctgt 421 gatgactgta tcctcttctt gggttgctgg gccccagccc cctttgccct agccccgggc 481 ttcctcctct tcccaggagg acccccttgg ccagccttct catccatttt cctgttcctc 541 ttcctcttcc ttcctgggcc tgggtctgca cttgaggatt ctgcctcact ccccgcttcc 601 ttattctggg gctgaggttt gggcaagcgc ttctttttct tcttccgttc ccgcctccct 661 tccagcatct tcttgtaggc tttttcaggg acatcatcct ctgggaaagt gcctccagcc 721 agctcccgca gct //