LOCUS BG076259 729 bp mRNA linear EST 12-MAY-2010 DEFINITION H3157E07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3157E07 3', mRNA sequence. ACCESSION BG076259 VERSION BG076259.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 729) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG076259.1. Other_ESTs: H3157E07-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3157 row: E column: 07 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..729 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3157E07-3" /db_xref="taxon:10090" /clone="H3157E07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 191 a 179 c 192 g 167 t ORIGIN 1 ccattacaaa gaaggcttta ttctctggca ggtgaactca tgtaacacca accccagttc 61 tcgaggtcac actccactgc actgaccctt cagcagcaca gcttcctatt cctgacagac 121 acgggagagc cccaacatcc aaggtctttt cctcaaatac aagtctcagt ctcttcaaca 181 atccattaga agtcatggct ctttaagagt tttgcacagc catgcaggag gcccagcatt 241 tgtactgcat ccatgggaag gcagaccagt agtcttgcct tgtgggatgg ctcaatagtt 301 agtacttgca ggttagattg tcaggagggt ttaggccagg accaagggct cagctccccg 361 ggctcccaag taggaaccat tctcccctcg ctgacactgc ttcctttcgc acaagtctct 421 tcttgtcgtc cagaggcttg gctaaggctc ggatgacctg agatttgtat ggcagtagca 481 cagaagtggg gaggcgagtg agagcgtgca tacactgcag agcagcaatc cggacagcca 541 ttgagtaact ggagctgagg ttcaggaatt ttgtaaccag agtgtcaaca tgaagactca 601 tgatttgagg tgcttccagt agtaggggtt ggagacagct cagagtggaa agctggacca 661 cagagtcagg gcagaataat gcttccagca gcaaggaaag aagtgtgggc agctctggta 721 aaagcacag //