LOCUS BG076259 729 bp mRNA linear EST 12-MAY-2010
DEFINITION H3157E07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3157E07 3', mRNA sequence.
ACCESSION BG076259
VERSION BG076259.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 729)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG076259.1.
Other_ESTs: H3157E07-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3157 row: E column: 07
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..729
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3157E07-3"
/db_xref="taxon:10090"
/clone="H3157E07"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 191 a 179 c 192 g 167 t
ORIGIN
1 ccattacaaa gaaggcttta ttctctggca ggtgaactca tgtaacacca accccagttc
61 tcgaggtcac actccactgc actgaccctt cagcagcaca gcttcctatt cctgacagac
121 acgggagagc cccaacatcc aaggtctttt cctcaaatac aagtctcagt ctcttcaaca
181 atccattaga agtcatggct ctttaagagt tttgcacagc catgcaggag gcccagcatt
241 tgtactgcat ccatgggaag gcagaccagt agtcttgcct tgtgggatgg ctcaatagtt
301 agtacttgca ggttagattg tcaggagggt ttaggccagg accaagggct cagctccccg
361 ggctcccaag taggaaccat tctcccctcg ctgacactgc ttcctttcgc acaagtctct
421 tcttgtcgtc cagaggcttg gctaaggctc ggatgacctg agatttgtat ggcagtagca
481 cagaagtggg gaggcgagtg agagcgtgca tacactgcag agcagcaatc cggacagcca
541 ttgagtaact ggagctgagg ttcaggaatt ttgtaaccag agtgtcaaca tgaagactca
601 tgatttgagg tgcttccagt agtaggggtt ggagacagct cagagtggaa agctggacca
661 cagagtcagg gcagaataat gcttccagca gcaaggaaag aagtgtgggc agctctggta
721 aaagcacag
//