LOCUS BG076192 524 bp mRNA linear EST 12-MAY-2010 DEFINITION H3156F12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3156F12 3', mRNA sequence. ACCESSION BG076192 VERSION BG076192.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 524) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG076192.1. Other_ESTs: H3156F12-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3156 row: F column: 12 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..524 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3156F12-3" /db_xref="taxon:10090" /clone="H3156F12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 156 a 104 c 97 g 167 t ORIGIN 1 aggaagacaa aaaacgtttt attttaaaac attgaaaaaa caaatgtctg ttatattgga 61 aatgtcaaat atgaaaactg tgttctatga tacacactcc cagatagcta aatactgtaa 121 attgtcctat catttccgct gttatcaaca cttgataaag ttggagatat accaaaggtc 181 tgctaatcat tattcaaagt catcatcctt gggttcaact cttttacaca agtaaaagtt 241 ctgtccagga tgttcctgac acatgaagct tccaggtgaa tttcagaaat attaagaaaa 301 gtatcttcct tttttgcctg tgaatgtttg cgtattgctg tactgttggc ttatatccac 361 tacagacact ggttctaggc cagcccaagg gtcttcaagc attgaaggct taaaataact 421 ttccaactca ttagacattc ttttttctct accacgccct gatccaaatg gtgtagatgt 481 ccttggagaa ccctgggggt aggtctgctg ctgccctggg gagt //