LOCUS BG076179 674 bp mRNA linear EST 12-MAY-2010 DEFINITION H3156E08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3156E08 3', mRNA sequence. ACCESSION BG076179 VERSION BG076179.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 674) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG076179.1. Other_ESTs: H3156E08-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3156 row: E column: 08 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..674 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3156E08-3" /db_xref="taxon:10090" /clone="H3156E08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 177 a 163 c 167 g 167 t ORIGIN 1 gtgagttaat taagtcgttc attgagggct tagttttcag agacaagaaa aaacaaactt 61 caccccttaa gcagatacac tccactctac ccaattatct gtaacaaatg cttcatctca 121 aaaaaccata gaccttggct cctgccaagc ttaagtgcag gaaacagcga gtaatccagc 181 tcttctcttc tagcccaagc cctagtcagc aagacggttg cagatctcgg agacagacat 241 ctgcggtgag gaaggcagct attgaagttc aggagcaaga gacagtttgc agtcagtgct 301 gtcagttccc ttttctactg atgtcttctt ccttcagggc aaacttcctc ctgaggacct 361 cagagatgag gacagcaggg tcttcttcct tcagtaaact ccggctcagg tcttggctct 421 gttttatccg gtgaaaatcc ttcaggatgc ccatcatgtc tgcaaagctg ctggccttgg 481 acagagagat gcagtcatcc tcttcagatg agctgcaggt agttttgtgt tctggtttgc 541 aacactcagg gctggcagaa caaagtaggg ggacggttgg gagctcaggg acctccacct 601 ccgtctcctc ggtgatgtca ctaatgacaa aggaagttgt agagtggagg gaggacccaa 661 agcaaggcaa aggc //