LOCUS BG076086 669 bp mRNA linear EST 12-MAY-2010 DEFINITION H3155E02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3155E02 3', mRNA sequence. ACCESSION BG076086 VERSION BG076086.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 669) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG076086.1. Other_ESTs: H3155E02-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3155 row: E column: 02 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..669 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3155E02-3" /db_xref="taxon:10090" /clone="H3155E02" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 130 a 192 c 183 g 164 t ORIGIN 1 cttcagaagc attttattgg gcaaagtctg aacaccaagg cgggctgcta tagaggtgtc 61 taacccagcc cctgggatga agttgtcccc tcacttccta gtttggctcc tggcttccta 121 ttggggccga ggtggctctt ggctcttccc aggctcctgc cgtctcagag tcatgagctc 181 ctcaaacatc catataagca tgggagtcag tacatggagc agtaggaatt gcaggtccag 241 catagcttca aacaaaggtg gcatggtgtg tgctggtctt gggctttgtg ggctcacctg 301 ggatggtgct caggccagga ccatttggta aggagggtcc attggctttt accctccttg 361 gacccggctg taccccgaga atgctgcctc cccacccagg agacacaccc gaaacgaggt 421 ccacatcgat gatcctgttg accgggaggc ctccgctgac tcgggccccg ccccatagcc 481 aagtggtttc tggggctgga gttcacagct gagttttgtt tgacaggagc acctttctgt 541 ttgtctgtgg acactttccc tcgagaagga tccctctcct ctaggatgac ccgttgtcca 601 tccagacttg agataggggc acaagatcca ggggctcctt ctccccgctc agtctcacca 661 gcaggctac //