LOCUS BG076062 861 bp mRNA linear EST 12-MAY-2010
DEFINITION H3155B09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3155B09 3', mRNA sequence.
ACCESSION BG076062
VERSION BG076062.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 861)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG076062.1.
Other_ESTs: H3155B09-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3155 row: B column: 09
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..861
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3155B09-3"
/db_xref="taxon:10090"
/clone="H3155B09"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 261 a 183 c 204 g 213 t
ORIGIN
1 agagtttatc tgatttttaa tttaattttg tgcgtgtgta cactaaacgc aaacccagct
61 ttgtacactc aagaccaaca ttcaatgcca ttagccctac ttcttcattt gtgaaattaa
121 gattctgtgt aaactttacc aagtaaccct gacttcaagt caaagagaga agtgtagggg
181 agagagtggg aaagtgctag gctaagggga tggaggggaa aagagccacc ttttcagagt
241 atggcttcac tctagtggac acctccagca accccacagc gctctcacca tctccttcag
301 cacccctcgc tcccctgctt tgctctcctg cttcggctgg atgtcagggc tggatgatgt
361 cagagctggc acctgcagct gctgagtctc attagaaaca agcgctgcct caacaaagcc
421 tgaatccagc ctaactcctc acactaccaa tgaggaaact aacactcaat gaggttgaat
481 aatttgtgaa gttgagttta gggcttcaag actcatataa gtgttgcatc tagggcaatg
541 gaattacatt taatgaaata cggcttgtta aaaagtaatt acacaaataa aggttctatt
601 aattctatct taggtaagct ggctgtagtg gtgcactcta gtaatcctag acctggaaga
661 cttgaggcag caggacggct gtgagctgtg ggcagcatgg catcctgggc cacagagtca
721 gaggatccta aatcacaaaa caaaatttac cttagggaaa aagacaaagt caccaaaata
781 agaaactaat aggcacgtaa ggccagtgat gtgatggtgt cgaagtagtc ggagaggaca
841 gataagagac atgcgagggg g
//