LOCUS BG075843 745 bp mRNA linear EST 12-MAY-2010
DEFINITION H3152D12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3152D12 3', mRNA sequence.
ACCESSION BG075843
VERSION BG075843.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 745)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG075843.1.
Other_ESTs: H3152D12-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3152 row: D column: 12
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..745
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3152D12-3"
/db_xref="taxon:10090"
/clone="H3152D12"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 234 a 135 c 135 g 241 t
ORIGIN
1 aaggaataac agtgatctcc aaaatgtctt ttttttctct aatcttaaaa tgggaagata
61 ccatctggag tggaatccta ctgtttattt ctcttgacct tatgaaaaag gcttgtattt
121 ccaaggccac cctgatggga tccagaccac cagcaagatg aatgtgtgac ttggaagcca
181 ggcactaaga ctgacaaaaa caaacataaa cattcttcat cagatacata gatgtattta
241 ggatggctaa ggcactacta ttcaaaaact atagtttttg cttgatttgc atttgttaac
301 ttctaacttg agcacaagtc tcctttgaat gctacttggg cactataaat tctgtgcaca
361 tcataaagtg tttctgtgag aaacatagct tgttggcctc tacaataaca aacataagaa
421 caaaaaatgt ctcacaacta tgttaactga gaaagtcatc cttcaatgct aattatcaaa
481 gctaatttaa ctattaataa gttttggatt agttgtggca atttcttctt tggggcaatt
541 ttagtatttc ttccatttga actttccgtt ttgttcattg agattacatt tgaaatggca
601 gggcttgacc tcagccatgg aaaaagcaag gctttgatta gttaatctga aaggaatcca
661 cttgggctat gtttttgtta ggcacagaca gaacttctag tggttaatgt aactctccaa
721 agtgcagcta acaaacgact gctgt
//