LOCUS BG075842 745 bp mRNA linear EST 12-MAY-2010
DEFINITION H3152D11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3152D11 3', mRNA sequence.
ACCESSION BG075842
VERSION BG075842.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 745)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG075842.1.
Other_ESTs: H3152D11-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3152 row: D column: 11
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..745
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3152D11-3"
/db_xref="taxon:10090"
/clone="H3152D11"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 182 a 173 c 151 g 239 t
ORIGIN
1 ggtcaccaac actattactg taaatgtatt ttttgttgtt tttgaagttt acaagctatt
61 aatgttatac atgtaagttt gaaggagtca taaacaacat ttattacctt agtatatcat
121 actggtcttg ttgctgttcc ttcacatttt cgaggttttc cattgccctc cctcccatag
181 tctgacaaga caatacattc ttgaactgtt agcccacagc agcaacacat attcctatgt
241 aaaaccattc atccagagta agatgctggt ccacaaggct ttccctacag aagttcaatg
301 gtgtcgaaag aatttgtaat acaccagacc gaccaggatg gctagctcca gcaagatgat
361 gacggagagc agcaacttgt tggttatcac ttttctggac attgagcgaa gaatcttccg
421 gcttttgctc aaattttcat ttgtatttac cagtctgccc ttagtacgtt ccaactggtc
481 tcgttgctcc cccaactctt ctatgatttc tgtaccaatt tgatcagttt ctgtggcaat
541 ccgatgagaa cgctcaatgc tttgggtagc ccggttcagg ctttcagtgc cttgtaggag
601 taatgctctt tgagactgta gtcgattcaa atgctcgttc tccaaggtat acgtgccata
661 cttcaggtct cctcggcctc caggagcggc tgtcagaggt gtacttctca cctcacggtg
721 gagtttagca aggtccttcc ggtag
//