LOCUS BG075826 590 bp mRNA linear EST 26-JAN-2001 DEFINITION H3152C07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3152C07 3', mRNA sequence. ACCESSION BG075826 VERSION BG075826.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 590) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3152C07-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3152 row: C column: 07 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..590 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3152C07-3" /db_xref="taxon:10090" /clone="H3152C07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 209 a 133 c 142 g 106 t ORIGIN 1 gcgtaaaaca agtttaattt ccaaccgggg cacagtcatt gcattatccc acatctttga 61 gcaaggatag agaaggcaag ttattaaaca tgtacagtct acattccaga gaaagcaaaa 121 agaaaaaaac ccacttcaga actgcagata ccactgtaac accacaaact ctggaggtgg 181 agggaagggg aagtccccac aaagaaaggt agaacaagat aaaaaataaa accagcttga 241 gaaaagcaag agagggggtt cacagaaatg ggatgaaaca cccaacccta aagatggatt 301 aggggatgta gaaagcaccc ccacctcccc acaaagccag ggatgttacc aaggtgctac 361 tgctccctgc tggatgtgtc tggagcagca tgcaccaagg caacattcaa ggctgcttta 421 gcctgctcag cctcaggttg gtcccacaag gtggagaaaa ataagctctc gtaaggggtg 481 caggagtgga ctcatgtctg caatgtacaa aacctatctg gaactagcat ctaagcagga 541 aggtcacaaa gtgtggcaga gcaaaaggcg aagtcctcac caatctcaaa //