LOCUS BG075740 645 bp mRNA linear EST 12-MAY-2010 DEFINITION H3151B03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3151B03 3', mRNA sequence. ACCESSION BG075740 VERSION BG075740.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 645) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG075740.1. Other_ESTs: H3151B03-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3151 row: B column: 03 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..645 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3151B03-3" /db_xref="taxon:10090" /clone="H3151B03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 128 a 168 c 197 g 152 t ORIGIN 1 gcggtttgat ctcagtagtt tattccggag acaaagcagt gcaagaggcg gccacgcttc 61 ccaccccctg ttttcagggt caggcctggg aaggtgactt acagacatca atcagttcaa 121 cactcaggtc ctgtaaacgt ggttagtcct ggggctctgc ttccgaggaa gccaatcggc 181 ttctccttgc ccaaaggatg cccctgcatg gcccttagtg gccttcccag ggctgctgct 241 tctagtgtac tctatcttat tagacctaag gacaacccag aggcctcatt ctctttacat 301 agtgagctga tgtcttaaga cacaagatca cccttggggg tcctgggctg ctgacctggg 361 gctccacagg ggtcctttcc tgtgggcttt ggggccagtg ggtggtctgc cagctgctgt 421 ggggtcagag tgagtctgaa atggggcaca actcccttgg gggcttgcct ggtgctcagt 481 gcaagggact cgctatgaac acagcaggaa ggagctgggc cttgctgttc caggaggggc 541 tgtcgcgtgt gaacaggagc agccctgtgg gtggtctcct ctcacctgaa aagagggacc 601 ggatgatgac atgaggaagc agagatcctc gtgggctaga ccact //