LOCUS BG075637 672 bp mRNA linear EST 12-MAY-2010 DEFINITION H3149F05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3149F05 3', mRNA sequence. ACCESSION BG075637 VERSION BG075637.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 672) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG075637.1. Other_ESTs: H3149F05-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3149 row: F column: 05 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..672 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3149F05-3" /db_xref="taxon:10090" /clone="H3149F05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 169 a 156 c 184 g 163 t ORIGIN 1 atagcagctt agagttttta ttaaaacaat cacttaaatt ataatcaccc caacccccag 61 aaaccagctt catgtcctgg ccccgatagg agtgacagga gcctcacatg aagagctgtt 121 tgaaaagagt aacagaggaa tggaagccca tgggcactgg cgtggcctca aggcacctag 181 gagagtctgg gtcctctgtg aaggctgctt gtaccaggga ctttgaggct ctggccctgt 241 tggcaggaac ggttagtcac tgttgctggg agtcagcctc tgggcttttt gaaagtatgg 301 gagcatgaga aacctgtcta aaagaggata tagcagtaag tgaagcccac tggtgttgtc 361 tagcaggggt tggcagtgac ctcaggtcag agtgctgtgg cggctcaggt actgcctcaa 421 tcagggtttg tgaccagcca caggcaagag caaggcctca gtgtcctgca ctgagagtga 481 cttgctgttt cagtagcagg gtaccctgcc tatgcttcag aaaccattga aactaggaag 541 agccagggtt gggcctcact ttggacttca gatgtttctc tttgactaaa attttctacc 601 agtaagaacc cagctacagg ctcaaagcag gctggagaca ttctggggac cattcgagct 661 ctctctctct ct //