LOCUS BG075613 765 bp mRNA linear EST 12-MAY-2010 DEFINITION H3149D03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3149D03 3', mRNA sequence. ACCESSION BG075613 VERSION BG075613.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 765) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG075613.1. Other_ESTs: H3149D03-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3149 row: D column: 03 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..765 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3149D03-3" /db_xref="taxon:10090" /clone="H3149D03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 172 a 188 c 202 g 203 t ORIGIN 1 ctggaacaga agtggcttta ttctgggtcc tgtcccccaa gctagctgga gtgctaaagt 61 gagaagagag tcctacagac aagctgagca aaactgtagg gatagctaag cctccacagc 121 ccgtccatta atgatgcgga tatgacgaat gatgtcctgg atggcttggg atgtggtgcc 181 ctggcctcca atatctgggg tatgcatatt ttcattgtcc atggatgcta agacagcttt 241 gcggatggaa gtggcatagg agtggagctt gagatggtct agcatcatgc agcttgctag 301 cagtgtggca gtcgggttag caatgttctt attggcaata cttttgcctg tgttccttgt 361 agctgtctcg aatactgcat acacatggcc gtagttggcc ccagccacaa ggcctgggcc 421 tccaactagc cctgcacaga cgttgttgac aatgttacca tagagattag gcatcaccat 481 gacatcaaac tgctgaggcc gggataccag ctgcattgtt gtgttgtcta caatcatgct 541 gtcaaaggtg atctgagggt agtgggctgc tacttccctg cagcactgga ggaagagtcc 601 atcacccagt ttcatgatgt tggccttgtg cacagccgtc actttcttac gcccactctc 661 ctgggccagc ttgaaagcat attcagcaat gcgcagggac ttggctttgg tgataatctt 721 caagctctcc accactcctg ctacgctctc atgctccagg ctgct //