LOCUS BG075581 700 bp mRNA linear EST 12-MAY-2010 DEFINITION H3149A04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3149A04 3', mRNA sequence. ACCESSION BG075581 VERSION BG075581.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 700) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG075581.1. Other_ESTs: H3149A04-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3149 row: A column: 04 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..700 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3149A04-3" /db_xref="taxon:10090" /clone="H3149A04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 180 a 216 c 163 g 141 t ORIGIN 1 atcacctatc agcgcatgtt caagagacaa ccacaaacac accaccactt ttagctatga 61 caatatgaca catttggcag cgttcacaga acagccttcc tcaccccaag ccattacaga 121 ggcccacaaa gtcctttctc tttggtccag ttcaagaagc tgggggagga agacggggtc 181 aaccaggtag gaaaaggctg cagaagcagt tacagcaact gctcagtccc gatgtccatc 241 tagcacggct taggatgccg cagcctcaaa cctctgggtg aggatgggct acgaggggcc 301 tggctgagag acaccaggtg atccactcgt gggaccagga acagcaactc ccacgctcca 361 accacactac aggacacctt atcctcctgc ctctgaggct ctgttcatag gattgtgggt 421 ccttcagcaa ctttgttctg tacccagaac ttgggtccac cacactaaca cggaatgccc 481 tgcccaggga agaatggtcc aagcagctgc tcatgtccat catggcgcac gcaggctgag 541 accaggcaca gctcaggaca agccttcttc ctcggctcag gcttttagaa aagcatctag 601 actgttccag ggagctagac cttactcctt tccccccaac tccccacaag ctccagccag 661 ggacccatcc cagatgccgt tgtgaaggtc tgttctaaca //