LOCUS BG075553 546 bp mRNA linear EST 26-JAN-2001 DEFINITION H3148F04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3148F04 3', mRNA sequence. ACCESSION BG075553 VERSION BG075553.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 546) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3148F04-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3148 row: F column: 04 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..546 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3148F04-3" /db_xref="taxon:10090" /clone="H3148F04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 177 a 116 c 84 g 169 t ORIGIN 1 ctttctttct ttctttcttt ctttctttct ttctttcttt ttttctttct ttctttaagg 61 tttcctccaa gtgatcatgt gacaacagat tagatacagt atggacatat caatatatac 121 ccagatttag cttaatagag gcatacggag ttacattgta actatctatc tatagtgtag 181 ttatttgtat gtatacaagt gaatatacta catatgcact gtcctttcac tcagctgaaa 241 ggcctagaga caacagcatc ttgtcagtga gcattcattg acaatgcaaa ccaagtgccc 301 agatcttggc ttctaatccc atcttccaat aaaagaatca gaagtcttta gaaaaactgg 361 ttgagtctag cactggatca ggaaatatac aagataagcc tagaatatat gttgtatgca 421 cacacatgca atcgtgcagg tgtgtataca cacatacaca cacacacaca cacacacaca 481 cacacacgat gaaatacatc aaaagcacat aggagtcaac tgaaagttct cccagtaatg 541 aaagct //