LOCUS BG075312 723 bp mRNA linear EST 26-JAN-2001
DEFINITION H3145G08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3145G08 3', mRNA sequence.
ACCESSION BG075312
VERSION BG075312.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 723)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Other_ESTs: H3145G08-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3145 row: G column: 08
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..723
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3145G08-3"
/db_xref="taxon:10090"
/clone="H3145G08"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 147 a 225 c 170 g 181 t
ORIGIN
1 ctcaaaacat atatttacct ccccactcag tacaccttcg ccacctctgc cagaacctcc
61 ctggaatgtg tagttcaccc acccagcatc ccatgccctc cctagccctt ggtgacaagg
121 cctttcacag cttgcacaat ggcgtccttg tcaataccga acatcttcag tagctcagct
181 ggcttgccac ttcgtggtac ttggctgaca gccaggcgag tgaccgtcac tccaggttca
241 cccactacgg cagcagacac tgcctctcct atgccacctt cgtagtagtg gtcctccacg
301 gtgaggatcc tgcctttggt tgctcgggca gagtctagga tgagtttcct gtccaggggc
361 ttgatagtga agggatccag cacccggatg ctgatcttat ctttctttag actctctgca
421 gcagccaagg cctcatgcag agttacacca gccccgatca ctgtcacttg gtcatccttg
481 ctcttcagga ccaccttggc ttggccgacc tggaaatcct cattgttgct ataaataatg
541 gcattctctg ggcggctggt ccggatgaag caaatgccct ttgtgttggc tgctaactcc
601 actgccttct ctgttgcaac tccatcgctt gggtaaaaga cggtggacat ggggactgac
661 cggaacatgg ccaggtcttc gagggccatc tgagagggcc gtcttcccca atggacacac
721 cac
//