LOCUS BG075253 553 bp mRNA linear EST 12-MAY-2010 DEFINITION H3145B04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3145B04 3', mRNA sequence. ACCESSION BG075253 VERSION BG075253.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 553) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG075253.1. Other_ESTs: H3145B04-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3145 row: B column: 04 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..553 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3145B04-3" /db_xref="taxon:10090" /clone="H3145B04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 151 a 136 c 124 g 142 t ORIGIN 1 ccttgataaa tggtttgttt atatccatta ctccccaata caggtaagtc atatagagta 61 ctagcaaaaa cgttccagtc agagctctca caaaagataa tacagagtgc tcagttgagg 121 ggcagatagc atgcccagag cataaagtct aaatataatc ttagtatata cagaagactt 181 ctccaagact cttcagcttt actgaggcat ctataaggac gtactcgggt ctgtctccct 241 tgccgccttc ccactgggga aagccgccca tgcctcagcc accaagttcc cactctgaaa 301 agtattaaaa actagccagg cgggcatgtc cagtttgagc agactgttga ccagtgttca 361 ttcctagata aggatgaccc tgagccttgc caaggtcaca cggctgcttg tgctgagttt 421 cagcacttac cttactggta gagtcacttt gctttgccag ccttagtcta gagggaaact 481 ggaagtagct ggggaaaaag cagcttgggc taggaccagc attgcgcagg catttcctgt 541 attaccagcc ttc //