LOCUS BG075166 570 bp mRNA linear EST 12-MAY-2010 DEFINITION H3144A07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3144A07 3', mRNA sequence. ACCESSION BG075166 VERSION BG075166.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 570) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG075166.1. Other_ESTs: H3144A07-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3144 row: A column: 07 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..570 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3144A07-3" /db_xref="taxon:10090" /clone="H3144A07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 151 a 137 c 125 g 157 t ORIGIN 1 ggttttttga gacagggttt ctctgtgtag ccctggctgt cctggtactc actttgtaga 61 ccaggctggc ctcaaactca gaaatccacc tgcctctgcc acccaagtgc tgggattaaa 121 ggggtgtgcc accatagaca agacgtttct taaagagaac agatacatcc aagttcttac 181 aaggaagctg catgtcaacc gcgtataaca gcgttttccc aaacataatc agtttaggtc 241 tagttaataa gattttcagt tagtgtcctg gaaaataaga gaatagactc aagataaggg 301 acaactgtct tcgctgtgtc acctagagag catctctatg gtgcccagtt tgacagtgaa 361 tatggaagga ggcttttggg tcctgaatct tacaagttcc atcattctgc agggaccatg 421 gctctgcagc cagtctgcca cgcacattag aggcatcaag tgggttctca cctagtgtat 481 cccagtgtag tcaacacaca cactgtcacc attagggcca cccatcagta tgctctatac 541 tttactcagt aacttgcttc tccattgtat //