LOCUS BG074854 558 bp mRNA linear EST 12-MAY-2010
DEFINITION H3140B11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3140B11 3', mRNA sequence.
ACCESSION BG074854
VERSION BG074854.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 558)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG074854.1.
Other_ESTs: H3140B11-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3140 row: B column: 11
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..558
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3140B11-3"
/db_xref="taxon:10090"
/clone="H3140B11"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 200 a 116 c 119 g 123 t
ORIGIN
1 aggaattcac cctaatggta aatatccaac aatttaaaaa atatataggc atgcctgaga
61 aaaatcactg tggcgctgtc tatcactaca aaatgtaaga cgctattgct aaggtcacag
121 atgtggaaga gtgaagagat gcacaacaat catgtagtgg aagccgtaaa aaccaaaagc
181 aaaaggcagc acacacccca caaagactca tacagtgatg tccagaatag tactgaagga
241 gggggaagag caaaaggtat agtaacgcta tacaaccttg taagacaggg aaacgcatgt
301 gcacctctgt gtgcacatac atgcctacct atgtgtgcac atacatgccg acctatatag
361 ctgctactcg cgtcagtcag aagtgtaaga agcaggtccg tattgaatgg taggttacag
421 cagaagggac acttctcacc tgactatctg tgtatacagc tccgacttct gactcattgt
481 aatagtttca ccaacttaaa aatgcaaagg agatgtcgaa gataaaatta aatcgaaaac
541 tgacactgct caactcag
//