LOCUS BG074752 761 bp mRNA linear EST 12-MAY-2010 DEFINITION H3138H02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3138H02 3', mRNA sequence. ACCESSION BG074752 VERSION BG074752.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 761) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG074752.1. Other_ESTs: H3138H02-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3138 row: H column: 02 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..761 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3138H02-3" /db_xref="taxon:10090" /clone="H3138H02" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 240 a 164 c 155 g 202 t ORIGIN 1 gcattttctg tatcatatga aggataccta tcaaataaag attcaaaatg aagtatttct 61 aaatccctgc tagatatcaa catcaaacat gcgcttggtg ccctcattga tttcttcagt 121 agagaccgac aaaggaaagg ctgggcatgg ctgagcgtac ctctaatttc agtgctgggg 181 gtaaggggag atgggtattg agagcttgct gcccagtcag attagcctaa gcttcaggtt 241 caatgagaga ttctgcctca aaaactaatg tacatagaag agaaagactc cagatgactt 301 ctaggttcta catgcacaca catgaatacg tatgtttaca cgcaaagaca tacacacaac 361 atattaaagg tcattaaaag cagtcattca cctagtttcc atacagttaa aactcatgtt 421 gaataactaa aatcagaaaa agctaaacac agcattattc aagtcgaaat agatatttct 481 ttacccttct gatttctgct gtagagaatc cagatgtttt gagctgctca cactctttat 541 gcagagtttt aaaggcttcc atcagctctt catactggaa aggaagtcag caggttagca 601 gttgcagcgc catccagcag tgtccttgtc accaaaccac ccactccctt gcaccaatca 661 cttcagtcct tggaggaaca gcactaaaaa cagtatggtg catgggcatc attctaggcc 721 ctggaaggct gaggcaggat ctcgagtgtg aattcagaat g //