LOCUS BG074722 665 bp mRNA linear EST 12-MAY-2010 DEFINITION H3138E06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3138E06 3', mRNA sequence. ACCESSION BG074722 VERSION BG074722.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 665) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG074722.1. Other_ESTs: H3138E06-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3138 row: E column: 06 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..665 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3138E06-3" /db_xref="taxon:10090" /clone="H3138E06" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 198 a 157 c 176 g 134 t ORIGIN 1 aaaagatatt aaatgctttt attttcaata ttaaaaaatc agtactttta atattaaaac 61 aactagtttt taaaaaaaga cagaaaagcc gaagtggtca tctacaggga agcccatcct 121 cactgccgca cacacacgtg cacacacacg cacacacaca cctctgactg acttcagtca 181 tgacgcccag cagtctctag tcacaggagc acacaataca cacaggccag gagcaagtac 241 caaaactgct ttagaaatga ctggccagct cagtccaggc gttagaaaaa tactctgctt 301 gaggagaaag gaagtacagt atggagtgca aaaggatgct cgcacccctc cggctgagct 361 tgaggctggg atgtgttcag ggcccagcat cggtcagcgg gtccgtgtta aggggcaagg 421 caagaccgac agatggtgac gtctaagggg agcaaaccca ggtcatgcct gcctgcgggg 481 taggcagccc ctttcagtga tggcccaaga tgggcttgct ggggagctag gagggtacca 541 agtagaagag ggtatggtta gttatctatg cccaatgtag gctgcagagg cactagctac 601 taaggtccag agagctccag tctccagcta ggctgggtcc atagcaagat acggtagggg 661 cacat //