LOCUS BG074613 653 bp mRNA linear EST 12-MAY-2010 DEFINITION H3137C01-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3137C01 3', mRNA sequence. ACCESSION BG074613 VERSION BG074613.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 653) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG074613.1. Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3137 row: C column: 01 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..653 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3137C01-3" /db_xref="taxon:10090" /clone="H3137C01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 203 a 160 c 120 g 170 t ORIGIN 1 cgggtgagtg ggagatggga agcctcaaga cagggtttcc ctgtgtagtc ttgtagacca 61 agccggcctt gaactcaaag actcacctgc ctctgcctca agtgctggga ctaaaggcat 121 gtgccacgac acccacatta acacttttgg tatcaatgag aggtaaagga tatcaatcat 181 attttcctga aaagaaaact acaattaaac tgctcaaggt caacaaagct agcttttctg 241 aaatcagggc tccctaactc aaatctcatg ttctctttac acataaacta ccagtggctt 301 tcagccaaac ccaaaactca atcttcaatt aattttaaga ccatttagaa actaaaagga 361 aatttaaaag gccttagaga aatcttaact ctctggtttg tatctaaaat taattgtcaa 421 agacatgcct caagccctcc atctggctcc tttccacaat aaggacctac cacagaagat 481 tccacgttac tacagaatac ctgctatcac atgggcctca aaccacttat ttccaagact 541 agattatgct ggctaactgc ttgtctttcc tgagaagctg ctaagagcag tcaagacatg 601 gctcccactt gtaagcaatg tgagctgggg ctgaagcctc tagagcacac ttc //