LOCUS BG074554 552 bp mRNA linear EST 12-MAY-2010
DEFINITION H3136E09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3136E09 3', mRNA sequence.
ACCESSION BG074554
VERSION BG074554.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 552)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG074554.1.
Other_ESTs: H3136E09-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3136 row: E column: 09
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..552
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3136E09-3"
/db_xref="taxon:10090"
/clone="H3136E09"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 179 a 101 c 96 g 176 t
ORIGIN
1 aatttttaaa aggtcgtttg ctttagttgt ttgttttttc aagacaaggt ttctcagtat
61 agcccaggct gtcctggaac tttagaccaa actggtctct aactcagaga tccgcctgct
121 tctgcctctc aagtgctagg gttaaaggcc tgagctatag tttgatggta agaaaatgag
181 taatttgaga gattaagcac aaaaatatat atcatctaga tataaatgtc agacaaaaca
241 ttataataac agaatctaat tcactttcat ttgggatccc tgatatgcag cacagtagct
301 gcacataaga ctcaaggaat gttttcagaa taaattaaat tacaatgaat gtatctttag
361 ttattcatta ctttaataaa ctgtattttt aaaaaaatcc cagcctcagc tctctcagga
421 gctactgata atgtttgcct ggggaatgaa agactttttt cagtagggtt gtcactgtaa
481 acgtttatgt tcctgaaaat aacccctcaa ccacattcct ataagtgacc tcagcaaaag
541 tgacctcagt aa
//