LOCUS BG074523 426 bp mRNA linear EST 12-MAY-2010 DEFINITION H3136B09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3136B09 3', mRNA sequence. ACCESSION BG074523 VERSION BG074523.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 426) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG074523.1. Other_ESTs: H3136B09-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3136 row: B column: 09 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..426 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3136B09-3" /db_xref="taxon:10090" /clone="H3136B09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 88 a 131 c 128 g 79 t ORIGIN 1 caaaggcaga cctgtggttt acttactctg ccaatcagct atctctctgg gcaagcccag 61 ggccaccctg cccagagggt cttccctggc cctggtccct tcccacccct aaaaggcact 121 gtaacaggtg gagggagtga agtagccgca gcaatggctg caaacatggt gccccagtga 181 agtagcctgg gggagcagga acaggagcaa aagtcaccct ggaatgccac cctggctgct 241 cagcatgttc gcacagctga ctagcacagg gggcagcatc cactcagagc aggaccccct 301 tgtgctggct agggcctcac ttgtcaaagt ggtccagggg gtagtgctcc ccgtaagtct 361 gcaggtgccg tgccagcgac tcctgctgcc tgcggagctg ggcgggcatc tcctggtaca 421 catcgg //