LOCUS BG074390 606 bp mRNA linear EST 12-MAY-2010 DEFINITION H3134E08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3134E08 3', mRNA sequence. ACCESSION BG074390 VERSION BG074390.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 606) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG074390.1. Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3134 row: E column: 08 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..606 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3134E08-3" /db_xref="taxon:10090" /clone="H3134E08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 166 a 151 c 129 g 160 t ORIGIN 1 agtagtcatc catcacttta tgacaggtct gtgtgaaatc tattcactat tagatgagct 61 agtctatgga ctcaccttat gcttacacaa accaaatggc ttaacttatt gcccacgtaa 121 gctctatgct agctcttata ctacaaacct gtccagcatg tcactgtact aaacactgtg 181 ggcacccgtc acaccattct gtctgtgtgt ccacacacat caaacgatgg aaaaggtcat 241 aggaagttgg caggtctgtc gagagcctat gggagcactg ttgacggaag tctttgcgtg 301 gcatttagaa gctggaaagg aaaagagggc agtggaatag agcaactgaa gaggaatatt 361 ggtcaactag tgtctcagaa tccaagagtg gagagctctc gaaagaagag ttgcccataa 421 tacctactaa ggtgctgctg acaagagtac ccttctgctg cctagttcct acttctcttc 481 agtctccgta cgcactcact agtccccagg aattcctctc tgtaccccca ctcctccata 541 ggtgtccacc ctcaagtgtc acaggagctt tgagcacttg ttccataata gcacgtcttt 601 ctaaaa //