LOCUS BG074313 623 bp mRNA linear EST 12-MAY-2010 DEFINITION H3133E09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3133E09 3', mRNA sequence. ACCESSION BG074313 VERSION BG074313.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 623) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG074313.1. Other_ESTs: H3133E09-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3133 row: E column: 09 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..623 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3133E09-3" /db_xref="taxon:10090" /clone="H3133E09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 161 a 155 c 181 g 126 t ORIGIN 1 gagaaaaacc gtttattcca tctccaaact agtctataga atctgagggc agggccatcc 61 ccaccagtct ccaatacatt cagtgcaggg gttcctgccc tccccactag tatctaaccg 121 ctgcttggta gtatttacat aaagctgggt gttatctcca ggcaaatcct gtccctcctg 181 ccaggagcgg gagaaagaag gaagtggcat cttgtccctc tgcctgtccc acggttggca 241 ggcagcctgg ggacaattgg cacagtggtg accccagaag acacagcaga gagctggctc 301 ctgaacctga gtgaggacgt ggggagacag gcacgaggat ccccaagctt ggctttgggg 361 aaagagttgg ggctcccagt ggagaaacta gcatgggtca aaaacatggt agcactaaca 421 ggcacctgct tctggctctc tgagaagggg atcggggtta ctaaaagcaa caaggagctg 481 ggcacacagc tggatggctg cgctggttaa gcaaatccaa gccctgggtc ctatctacag 541 tactatggtg ggggataaaa agatctagtc aaaaccatct caaaagtgcc cttgaggatt 601 gggtgggcgg cggcgaagcc cag //