LOCUS BG074273 641 bp mRNA linear EST 12-MAY-2010 DEFINITION H3133A12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3133A12 3', mRNA sequence. ACCESSION BG074273 VERSION BG074273.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 641) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG074273.1. Other_ESTs: H3133A12-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3133 row: A column: 12 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..641 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3133A12-3" /db_xref="taxon:10090" /clone="H3133A12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 133 a 167 c 156 g 185 t ORIGIN 1 cttttcaagc actattttat agaacatttt tcctgagtgg actccttgat gtttttatca 61 tctctcttgc tcttgtaagg tttaccactg ctgtggattc tgtgatgaat tataagattt 121 gaccgccaac caaagctctt accacacaca tcacacttgt aaggcttctc ccccgtgtgg 181 actctctgat gggactgtag ctgtgaagag cgactgaagc ctttcccaca cacgtcacaa 241 ctgtacggct tctcccccgt gtggactctc aagtggactt ggagatttga attcagagta 301 tagcctttcc cacacatagt acaaacgtat gatttctctc cggtgtgaat gccctgatgg 361 gcctgaagac tcgaagggcg actgaattct ttcccgcagt ccacgcattt atagggcttt 421 tctcctgtgt ggactcgctg atggatgtag agattggagc tacaaatgaa gctcttccca 481 cactccccgc acttgtatgg tttctctccc gtgtggaccc tctggtggga ccgcaggtgg 541 gaactgcgac tgaaactctt gccgcaggca tcgcatttga acggcttctc cccagtgtgg 601 agcctctggt ggtcctggag gtgggaggcc tggctgaagg c //