LOCUS BG074179 754 bp mRNA linear EST 12-MAY-2010 DEFINITION H3131G08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3131G08 3', mRNA sequence. ACCESSION BG074179 VERSION BG074179.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 754) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG074179.1. Other_ESTs: H3131G08-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3131 row: G column: 08 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..754 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3131G08-3" /db_xref="taxon:10090" /clone="H3131G08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 228 a 208 c 164 g 154 t ORIGIN 1 ctagaattcc cccttttaaa tctgtcatgc tgggcggttg tggagatgct cgcctttaat 61 cccagtagag gcagaggaag gtggatttga ctcaaaggtc agcccagact gatccaagct 121 gctctttccc caccctaacc agaataggaa gtctgcacac atctctctac tcacagggcc 181 agcttacaag gcctttcaaa cccagcaggc cactcaccca agcatggaaa tatataatct 241 caaaagttcc aaaggatgcc aactgtgagg ccacaccacg ttccttcaca gaggacacag 301 gccacttgtt atgacccccg gaaagcttta accactctag agttaaagtc cacaccaggg 361 ctgttttaag agcatctagc ttcctcttgc cacatacaac ccaactctca aacaatctac 421 caagcaattc aggagaacag aacagaggct ctggccaagc caggtggcaa aggcctgccc 481 caactactca ggaacctgtg gcaggagcct caggttcaag gcctcctatc tctagactaa 541 ggtgagttca aggcctacca ggacaacgtg gtggttaaga gacagtcgtg aacataaaaa 601 caaaggaccc aggggtggct cagtcccaca catagtagct caaggctctg gaatggaatc 661 tgcaatacca aaaccaacta accacaaagt gttgcggtgg tatcactaaa gctacgaact 721 ctcaggaaca cacaggtgta ttcacagtat ggaa //