LOCUS BG074127 590 bp mRNA linear EST 26-JAN-2001 DEFINITION H3131B11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3131B11 3', mRNA sequence. ACCESSION BG074127 VERSION BG074127.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 590) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3131 row: B column: 11 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..590 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3131B11-3" /db_xref="taxon:10090" /clone="H3131B11" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 149 a 172 c 142 g 127 t ORIGIN 1 aacgaagaaa atcccccatc ttttaatgca atcactttcc acatcccttc atttagaggc 61 acaaaggagc ttgtgggtca ggaagtctgc ctgtcagtca ggaagtctgc ctggcatgca 121 cagaaagtca ggcttcaatg ccagccacgc acccgagatc acgctaaatc ttcagaagcc 181 atctcttcgg gcctgtacta ttgttcagca aactaagtga agcaagtcct acacctgttc 241 cttcctgccc tgccaccccc accctcatct tagaagccct acaccccaac ttagtaacta 301 cttcttgtcg aggtagagaa cttccaggga accccacatt tatgaggggt catcaagatg 361 aggcagctct atcccatctg ggaggaagag gatggctggg cccctgctgg ctgtgcaccc 421 aggcctgctt cagactctgc tccctcccta gtccgcttcc acggacacag agaggctcac 481 atctgcactg aagtgggcga cacagaaatg cctgttgcaa tgagactgtg ggtcgcagtg 541 gctcgtcggg ggaagagggt agaaggcatc acactgcagt agaagcaaac //