LOCUS BG074107 660 bp mRNA linear EST 12-MAY-2010 DEFINITION H3130H12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3130H12 3', mRNA sequence. ACCESSION BG074107 VERSION BG074107.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 660) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG074107.1. Other_ESTs: H3130H12-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3130 row: H column: 12 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..660 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3130H12-3" /db_xref="taxon:10090" /clone="H3130H12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 152 a 175 c 148 g 185 t ORIGIN 1 gtgagtagaa atattttatt ggtgagaccc caccatctgc acaaagtggt cctggaatca 61 agctccttcc tccttggcaa tgcgatcttt cttgagtggt cccataaatg ctttcttctc 121 ctccatggtc tggaagcgac catggccaaa tttggaggtg gtgtcaatga acttcaggtc 181 aatcttctcc agggcccgac gtttggtctg aaccagcaag gacttacgaa gagtaagtac 241 tcgcttcttg gtccccacca cacagccttt gagcatgatg aagtcattgg tcacctcacc 301 ataatgcaca aagccaccca gtgggttgat gctcttgtca gacaagtcat agtcagtaga 361 tgcattgttc ttgatcagtt tgccatcctt gatgaggtag ccttgaccaa tcttgtaaat 421 cttcttgtta atctctgttc ggtgatggta gcctttctgc ccagctcgag ccacagagaa 481 ggcaacacgg gcagggtgcc aggctccaat acaggcaact ttgcgcaaac ctcgatgggt 541 ctttcggggc agtttctttg tatgccaacg actggttacc cctttgtacc ctttgccttt 601 tgtcacgcca atgacatcaa tcatctcatc ctgcccaaac acctggttca caggaacctg //