LOCUS BG073552 511 bp mRNA linear EST 12-MAY-2010 DEFINITION H3124A04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3124A04 3', mRNA sequence. ACCESSION BG073552 VERSION BG073552.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 511) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG073552.1. Other_ESTs: H3124A04-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3124 row: A column: 04 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..511 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3124A04-3" /db_xref="taxon:10090" /clone="H3124A04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 145 a 118 c 85 g 163 t ORIGIN 1 cattttttaa aaataaagtt tattagatca taatgttgtc tgaatttacc acctttttca 61 aaagtcagct cagcttccaa tgtagtctac aatccgttaa tcaaggccag cagcctatgg 121 atagcttcac cgtttatagt cggcttttca gtagccagac aaacttcccg aaatagctgt 181 aatgactgat tcatcctctc aaattctctt gcctttctgt gtcccttctg aataacctcc 241 tccgggagat tagcaagcct tgctgcatta aagccatagc tcttaggaca agctccctta 301 atgaatttat agaggaaagt aatagtctcc tggctggggt cctcacattc attttctacc 361 atgcatgcca tatgtcctag gcgcacacaa acacttttag aataatcttc tactaatgaa 421 tgatagtgtg tagaaaacag tgttcgacac tttatggttt caacaagttc tttaacaacc 481 gcattggcta tcgctgtccc atcaaaagtt g //