LOCUS BG073533 642 bp mRNA linear EST 12-MAY-2010
DEFINITION H3123G05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3123G05 3', mRNA sequence.
ACCESSION BG073533
VERSION BG073533.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 642)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG073533.1.
Other_ESTs: H3123G05-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3123 row: G column: 05
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..642
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3123G05-3"
/db_xref="taxon:10090"
/clone="H3123G05"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 175 a 152 c 120 g 195 t
ORIGIN
1 aaaaatataa aatcttgctt tatcgtcctt ggtttttctc aacaatttcc aaagaaaact
61 ggcttccccc actggtgtgg gtgcatccga ctgagatcag ggaagagaat taaaattgaa
121 cgacttgctt ttttctttct tgatttttct gaacaacttc catggaaacc tttgcctcat
181 acagtgggat gggctcatcg agcagaggcc tgaaaacacc agctgataac ttctccatca
241 catcttttaa gatacaaaga tattttccct gttgtgcatt tgacaaattc caaacttcat
301 cattaaggaa agccaccgtt gctcccttga ggaaaaggca gtggctatct ggaggatcca
361 actgaaggtt ttcttctgtt gttacccaat ggcctccgac accaagaagt gtgatgatgt
421 catgcttatg tggcagatgt aactttacag ctggttcatc atctttacta tataatctca
481 ctcctgcatc tatgacaata tctactccca ggccacctgt ttcctccaaa cagctttcag
541 caacatggac tttcccatta gacacatcaa tcacacgggc tatagaaggc ctaagccttt
601 caagatgttg cttgtcctcc aggctgtgtg ctgttgagat aa
//