LOCUS BG073463 705 bp mRNA linear EST 12-MAY-2010 DEFINITION H3122H05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3122H05 3', mRNA sequence. ACCESSION BG073463 VERSION BG073463.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 705) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG073463.1. Other_ESTs: H3122H05-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3122 row: H column: 05 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..705 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3122H05-3" /db_xref="taxon:10090" /clone="H3122H05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 177 a 187 c 177 g 164 t ORIGIN 1 aaaatatctg ttctaaagat gttgggatgt ccctggaagg ctatcaagac aacctcctca 61 cacctgactc cttccttcat cagcaagcag gccatgcttc ccagctccat acagagcacc 121 tgggcagtcc ctagcaaagg cttgcagaag cagttctgat ggtaccgttg tgagggtcac 181 ctaccgtaac agttacactg ggtgcgccac aaggtgttct caagactttt ttctgtgtga 241 gactcgttac tccgttcttc ccacacgatg gaaacctgca cccgggcaca agaaccagat 301 tatgagcaga aagccaaggt tctcctgttt ctcagctctt gggaaatggc acactgcatg 361 tggtggctat tgcgtcggca gcttggagca gcagtcttga gtcccctggc tctctgtaga 421 tgagcacaag gcgcgcatgg tgtgctcagc aggagggtga taccaggtgt aggctgtctg 481 catggctggc cttttaaacc tgccatcgca tcacatgtat tgtgaccttg tgctgaaagc 541 tacctgtcat ctccaatcag gtgacccaaa aaaaagctgc caaggcacca gtctcaacaa 601 gggttacagt ccccaagctc ctctggggaa gcgttcagtg acaggttaac ctaaaacatg 661 cagctcagga gcaaagtcac tgggacacca gcttgtaaaa ggaag //